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Abstract CRISPR/Cas9 assisted targeting scheme (CATsCH) is an in vitro method that targets long sequence specific genomic regions of interest. The RNA-guided Cas9 digestion system was adjusted to in vitro conditions in combination with cloning technique to facilitate the integration of long ...

The TechnologyEpigenetic profiling: single-molecule, quantitative optical detection by fluorescent labeling of 5hmC residues. A simple yet highly sensitive 5hmC quantification assay that is compatible with bulk DNA analysis as well as with 5hmC detection on individual DNA molecules. The ...