Our invention is a new method for the successful synthesis of Multi-phosphorylated peptides that applies different coupling protocols and paves the way for the efficient and routine synthesis of multi-phosphorylated peptides of biological interest.
Phosphorylation is a key regulatory process in the cell. Many cell-cycle regulatory proteins undergo multiple phosphorylations at multiple residues, leading to changes in their biochemical properties and biological functions.
Synthetic multiphosphorylated peptides are essential for studying the mechanism of action and regulation of multiphosphorylated proteins as well as the correlation between the phosphorylation pattern and the biological function of proteins. However, the synthesis of multi-phosphorilated peptides is limited.
We used this approach to synthesize a series of Rhodopsin derived peptides with up to seven phosphorylated Ser and Thr residues.
The peptides were obtained in high yield and purity.
Our results show that adjusting the coupling conditions based on the specific phosphorylation pattern is the key for the successful synthesis of multiphosphorylated peptides that could not be synthesized using any other protocol.