Reches Meital, HUJI, Faculty of Science, The Institute of Chemistry
The surface chemistry and topography of implants are very important to their design. This is because the interface between the implant and the human body will determine how well the implant would integrate in the desired tissue. Surface roughness encourages mammalian cells adhesion and therefore implant integration, however, this roughness also attracts bacteria and therefore assisst bacterial colonization. This colonization leads to the formation of a well-defined bacterial network termed “Biofilm”.
Biofilm formation on medical devices and implants leads to severe infection which may result in implant failure and even in patient death. Therefore, there is a need for a coating that will resist bacterial attachment and encourage cell adhesion.
This invention is a simple molecule, a peptide that consists of only 6 amino acids. It contains an element that resists bacterial adhesion, a motif that encourages mammalian adsorption and amino acids that encore the peptide to the surface and mediate its self-assembly into a coating.
We developed, manufactures and sells a novel coating which prevents bacterial adhesion to surfaces. The coating spontaneously formed by the self-assembly of a tripeptide. The advantages of using peptides for this purpose are concealed in peptides' biocompatibility, chemical diversity, and ease for large scale synthesis. The coating is formed by the peptide on various surfaces (glass, titanium, silicon oxide, metals and polymers). The coating completely prevents the first step of fouling which involves the adsorption of bioorganic molecules to the substrate.
· A simple molecule made of amino acids.
· The molecule spontaneously forms a coating under mild conditions (either room or body temperature) without any curing step.
· An agent capable of preventing or arresting adsorption of organic and/or bio-organic materials to a surface.
· An element that encourages attachment of cells to the surface.
The materials of the invention are capable of attracting cells from multicellular organisms and preventing antifouling by unicellular organisms. The materials of the invention can be used in biotechnological processes with any eukaryotic cells including mammalian cells or plant cells.
The invention provides a method for preventing bacterial contamination while the implant is integrated into the human body. The invention provides a method for prolonging the lifetime of an implant and for stimulating or encouraging bone healing or absorption of an implant after implantation.