Categories

Genetics, gene cloning, protein production

Objective/function

The facility functions as a resource and training center for scientists in the fields of gene construction and protein production

Research provided

* Specialization in four major expression systems for a wide variety of applications:

Bacterial cells: Optimization of expression conditions in a wide variety of vectors and E.coli strains
Insect cells: Specialized baculovirus facility, using the latest developments for optimization of expression for both secreted and non-secreted proteins
Mammalian cells: Production of high-titer lentiviruses for induction of primary cells and live animals
Cell-free expression: Production of up to several milligrams of proteins in E.coli and wheat-germ based lysates

* Individual training programs in the fields of gene construction and protein expression

* Annual graduate-level workshops in protein expression and purification

Advantages

State-of-the-art technologies, equipment and scientific support to all research laboratories at The Hebrew University, other universities and biotech companies
Implementation and development of technologies in gene cloning, protein production, and mammalian cells involving gene silencing, reporter genes

Client record

Available equipment

Educational website with bioinformatics tools, specific useful protein expression procedures and tutorials used as a support tool for students and scientists.
A wide range of temperature-controlled incubator shakers for bacterial expression. Capacity: 10 liters/week
Insect-cell incubator shakers. Capacity: 2 liters/week
ProteoMaster(Roche Applied Sciences) cell-free expression apparatus.
Standard equipment for molecular biology lab such as gel documentation station, spectrophotometer, laminar flow hoods (x3)

Staff

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